Structures

Selection of publications:

• Protein X-ray structures (with pdb accession codes)

PDB Structural Database

birefringent protein crystals

protein structures can be elucidated using X-ray diffraction data from protein crystals

recent structure:
GAPR-1 complex (4AIW)

GAPR-1n complex with lipid-binding inhibitor I6P

Schouten et al. Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization (2012) Biochimica et Biophysica Acta (BBA) - Biomembranes 1818: 2175–2183
 

Human complement factor B (2OK5)

Factor B

HEK293-S glycosylation

Milder et al. Factor B structure provides insights into activation of the central protease of the complement system (2007) Nat.Struct.Mol.Biol. 14: 224-228  

 

Complement component C2a (2I6Q and 2I6S)

C2a

Milder et al. Structure of complement component c2a: implications for convertase formation and substrate binding (2006) Structure 14: 1587-1597

Glutathione S-transferase (1M0U)

GST-2

Agianian et al. Structure of a Drosophila Sigma Class Glutathione S-transferase Reveals a Novel Active Site Topography Suited for Lipid Peroxidation Products (2003) J.Mol.Biol. 326: 151-16


Phosphatidylinositol transfer protein alpha (1KCM)

PITP-alpha

closed and open conformation of the PITP protein

Wirtz et al. Phosphatidylinositol transfer proteins: From closed for transport to open for exchange (2006) Advances in enzyme regulation, 46, 301-311.

crystallographic dimer

Van Tiel et al. The structure of phosphatidylinositol transfer protein alpha reveals sites for phospholipid binding and membrane association with major implications for its function (2002) FEBS letters, 531, 69-73.

Schouten et al. Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association (2002) EMBO J. 21: 2117-2121                                   

 play video above: switching between the open and closed structures of PITP

Leech-antiplatelet protein (1I8N)

rLAPP

Huizinga et al. The structure of leech anti-platelet protein, an inhibitor of haemostasis (2001) Acta Crystallogr.,Sect.D 57: 1071-1078

Beta2 glycoprotein I (1QUB)

B2GP-I

Bouma et al. Adhesion mechanism of human beta(2)-glycoprotein I to phospholipids based on its crystal structure (1999) EMBO J. 18: 5166-5174

• Other studies

L-amino acid methyl esters

 

X-ray structure of L-Serine methylester hydrochloride

 

Schouten et al. L-Serine methyl ester hydrochloride (2009) Acta Cryst. E65, o3026 (IUCr VM2012) 

 

Lutz et al. L-Alanine methyl ester hydrochloride monohydrate (2011) Acta Cryst. E67, o586 (IUCr EZ2229)

 

Study of microgravity crystal growth   

 

A new upside-down geometry is proposed to achieve the beneficial effects of microgravity crystal growth by making use of buoyant forces instead of compensating for them. 

Poodt et al. Simple geometry for diffusion limited protein crystal growth: harnessing gravity to suppress convection (2009) Crystal Growth & Design, 9, 885-888